RNA-seq of Bone Marrow Mesenchymal Stem Cells:adult female bone marrow

Total RNAs from aged BMMSCs (P10–12) with/without em-eNMs treatment were extracted by Trizol. Libraries were prepared using NEBNext® kit and sequenced on Illumina HiSeq 3000. QC used FastQC and FASTX toolkit. Data were mapped to human genome (NCBI build 37, mm9) by HISAT2. Three samples per group. DEGs were identified by Ballgown (≥1.5-fold, P<0.05). The study design involved extracting total RNA from aged BMMSCs (P10–12) with or without em-eNMs treatment using Trizol, constructing sequencing libraries via NEBNext® Ultra™ kit, and sequencing on Illumina HiSeq 3000. FastQC and FASTX toolkit were used for data QC, followed by mapping to the human genome (NCBI build 37, mm9) with HISAT2. Three biological replicates per group were analyzed for DEGs using Ballgown, with significance set at ≥1.5-fold change and P<0.05.