Data from: Natural thermal stress-hardening of corals through cold temperature pulses in the Thai Andaman Sea

# Natural thermal stress-hardening of corals through cold temperature pulses in the Thai Andaman Sea ## Description of the data and file structure This dataset includes all data for the manuscript "Natural thermal stress-hardening in corals: Cold-water pulses boost heat resistance" by Wall M./Roik A. et al. which investigated the phenomenon of stress-hardening through environmental priming in corals during two distinct seasons. Data were collected at the Andaman Sea coast of Thailand. This data collection contains environmental data (temperature °C, HOBO Pendant Temperature/Light 8K Data Logger, Onset, USA), coral bleaching score data (decline of coral tissue coloration, assessed as established in Siebeck et al., 2006), and the effective quantum efficiency (yield Φ PSII, pulse amplitude-modulated fluorometer Diving-PAM, Walz, Germany). The two latter variables are metrics that capture the coral stress response and were assessed during short-term heat stress assays (the mothofology is also described in details in Doering et al. 2021). The stress response data were the delta values of pre-heat stess and post-heat stress measurements. The stress response data were analyzed comparing between reef sites of origin and seasons using effect sizes (following the instruction to the R package dabestR v0.2.3 6 (Ho et al., 2019)). Additionally, linear-Model based analyses were conducted with the response data (following instructions in the package nlme v4 3.1-148 and lme4 v1.1-23, R). All analyses were performed in R studio. Visualizations were generated via ggplot2 and assembled in Adobe Illustrator. These figures are also shared herein. ### DATA FILE STRUCTURE EnvironPrim_Data.tar.gz EnvPriming_ReadMe.md \---EnvPriming_data +---Data \| Input_Data_delta_BSC.xlsx # Input data for downstream analyses. Contains the delta values of bleaching score averages calculated from the max. and min. values recorded at each asesssement time point. Delta values are mostly negative, i.e. = indicating coral bleaching and delcining health = stress response; the data is organzsed into 4 tabs by coral species and season; ; All acronyms in the headers and tabs are explained in the LEGEND SECTION below \| Input_Data_delta_PAM.xlsx # Input data for downstream analyses. Contains the delta values of the effective quantum efficiency (yield Φ PSII) measured by a diving-PAM (pulse amplitude-modulated fluorometer); the data is organized into 4 tabs by coral species and season; All acronyms in the headers and tabs are explained in the LEGEND SECTION below \| Rawdata_BSC.xlsx # Original collected data (transcript from underwater proof field sheets). Contains bleaching scores recorded during the heat stress assays, see score scala by Siebeck et al., 2006 (we use internsity scores 1-6). At each assessment the minimum and the maximum score of a fragment were recorded; All acronyms in the headers and tabs are explained in the LEGEND SECTION below \| Rawdata_PAM.xlsx # Original collected data (transcript from underwater proof field sheets). Contains effective quantum efficiencies (yield Φ PSII) measured by a diving-PAM (pulse amplitude-modulated fluorometer); At each assessment the 3 measurements per coral fragment were recorded; All acronyms in the headers and tabs are explained in the LEGEND SECTION below +---Effectsizes_output \| Fig_3_CB.tif # calculated effect sizes from dabestR are represented along a color gradient in this heatmap \| Fig_4_CB.tif # this figure is a composition of all "Cumming estimation plot" from dabestR sorted by coral species and season \| Fig_3-4_Tables.xlsx # the tabs contain: TAB1) raw output from the dabestR analyses (bleaching score data); TAB2) raw output from the dabestR analyses (diving-PAM data); TBA3) the output from Tab1) and Tab2) organized in a summary table; TAB4)summary table form Tab3) rearranged for plotting the values in heatmaps \| Fig3_Rcode.html # R code produces the elements for the heatmap in Fig_3, R code, versions and results \| Fig4_Rcode_bsc.html # R code for dabestR conducted with bleaching score data, R code, versions and results \| Fig4_Rcode_pam.html # R code for dabestR conducted with diving-PAM data, R code, versions and results +---LMs_output \| Rcode_MixedModels_bsc_poc_apr_Sep2021FinalV1.html # Statistical analysis of delta-bleaching score , R code, versions and results \| Rcode_MixedModels_bsc_poc_nov_Sep2021FinalV1.html # Statistical analysis of delta-bleaching score , R code, versions and results \| Rcode_MixedModels_bsc_por_apr_Sep2021FinalV1.html # Statistical analysis of delta-bleaching score , R code, versions and results \| Rcode_MixedModels_bsc_por_nov_Sep2021FinalV1.html # Statistical analysis of delta-bleaching score , R code, versions and results \| Rcode_MixedModels_pam_poc_apr_Sep2021FinalV1.html # Statistical analysis of Delta-Y (effective quantum yield), R code, versions and results \| Rcode_MixedModels_pam_poc_nov_Sep2021FinalV1.html # Statistical analysis of Delta-Y (effective quantum yield), R code, versions and results \| Rcode_MixedModels_pam_por_apr_Sep2021FinalV1.html # Statistical analysis of Delta-Y (effective quantum yield), R code, versions and results \| Rcode_MixedModels_pam_por_nov_Sep2021FinalV1.html # Statistical analysis of Delta-Y (effective quantum yield), R code, versions and results +---Temperature \| Fig_2_CB.tif # Timeseries plot of temperature records from the study sites; created in R environment \| Input_Data_Temperature.xlsx # In situ temperatures in the study sites RE and RW (one site presented per tab) recorded in Celsius using HOBO Pendant loggers \| Fig_2_Rcode_Temperature.R # R Code that generates Fig_2, R code, versions and results ### LEGEND POC = Pocillopora sp. (coral species) POR = Porites sp. (coral species) season 1 = April 2018 (season of internal waves peak) season 2 = November 2018 (season of internal waves absence) apr = April nov = November site = study site / reef location where corals were collected RW = Ko Racha West Shore 7.595530°N, 98.354320°E RE = Ko Racha East Shore 7.598910°N, 98.373100°E treatment = temperature exposure treatment of the short term heat stress assays (also see Doering et al. 2021) A = ambient temperature treatment of 29 °C (control treatment) H = heat exposure of 34 °C colony = indicated the coral colony number N = Replicate number tank = Experimental tanks "A1", "A2", "H1", "H2" deltaBSCboth = delta values of pre-heat and post-heat bleaching score measurements deltaY = delta values of pre-heat and post-heat measurements by PAM (i.e., effective quantum efficiency (yield Φ PSII) measured by a diving-PAM, pulse amplitude-modulated fluorometer) Y 1-4 = technical replicates of effective quantum yield measurements Y1, Y2, Y3, Y4 (diving-PAM) NA = value not available HM = heatmap IW = internal waves LMs = Linear Models PAM = pulse amplitude-modulated fluorometer Timestamp = date and time of temperature record from the HOBO Pendant loggers dd/mm/yy hh:mm Temperature = recorded in situ temperatures in Celsius ### REFERENCES Doering, T., Wall, M., Putchim, L., Rattanawongwan, T., Schroeder, R., Hentschel, U., & Roik, A. (2021). Towards enhancing coral heat tolerance: a “microbiome transplantation” treatment using inoculations of homogenized coral tissues. Microbiome, 9(1), 102. Ho, J., Tumkaya, T., Aryal, S., Choi, H., & Claridge-Chang, A. (2019). Moving beyond P values: data analysis with estimation graphics. Nature Methods, 16(7), 565–566. Siebeck, U. E., Marshall, N. J., Klüter, A., & Hoegh-Guldberg, O. (2006). Monitoring coral bleaching using a colour reference card. Coral Reefs, 25(3), 453–460. ## Authors:data preparation and data curation Dr. Marlene Wall, Dr. Anna Roik, ## Sharing/Access information The original preprint can be accessed from here: Supplementary material can be downloaded here: