Whole genome sequencing of SOD1 A4V iPSC and Zinc Finger Nuclease Corrected iPSC

Induced pluripotent stem cells were obtained from an ALS patient with an SOD1A4V mutation. To determine whether the phenotypes we observed were dependent on the SOD1A4V allele, we used a two-step, zinc-?nger nuclease (ZFN)-mediated gene targeting strategy to correct the mutation in iPSC line 39b. Stem cells were sequenced at 30X coverage to examine off target cut sites, altered copy number variation and karyotypic stability of the modified line.