The differential expression of tRF&tiRNA in eutopic and ectopic endometrial tissue of patients with ovarian endometriosis.

Endometriosis is a chronic gynecological disease that afflicts millions of women of reproductive age and has traditionally been diagnosed by laparoscopic surgery. This diagnostic barrier delays identification and treatment by years, resulting in prolonged pain and disease progression. Development of a noninvasive diagnostic test could significantly improve timely disease detection. We characterized eutopic and ectopic endometrium from five patients with ovarian endometriosis using tRF&tiRNA sequencing. We identified 294 tsRNAs by RNA microarray technique in eutopic and ectopic endometrium. Under the criteria of |log2FoldChange >2.0| and p-value < 0.05, 11 tRFs/tiRNAs were reported to be dysregulated in ectopic endometrial tissue compared with eutopic endometrial tissue, of which eight were up-regulated, and three were down-regulated. Using high-throughput sequencing, we detected the expression profiles of tRFs&tiRNAs in eutopic and ectopic endometrium tissues of 5 patients with ovarian endometriosis.