Aortic gene expression profiles of double ApoE and ApoA-I knockout mice

Eight weeks old double apolipoprotein E and apolipoprotein A-I knockout mice (DKO) were fed either normal laboratory diet (chow) or high-fat (Western) diet for 6 or 22 weeks. High-throughput sequencing technologies were used to analyse the transcriptome of the aorta of apolipoprotein E knockout (EKO, GSE163657) and DKO mice to showcase the use of the software reString (https://github.com/Stemanz/restring), a functional enrichment results aggregator. RNA-seq performed in mouse whole aortas from double apolipoprotein E and apolipoprotein A-I knockout mice (DKO), n=3-4 per experimental group, using the Illumina Genome Analyzer IIx. Two diets (chow, Western), two time points (6 weeks, 22 weeks).将8周龄的载脂蛋白E（apolipoprotein E）与载脂蛋白A-I（apolipoprotein A-I）双敲除小鼠（DKO）分别饲喂正常实验饲料（常规饲料，chow）或高脂（西式）饲料，饲喂时长为6周或22周。采用高通量测序技术分析载脂蛋白E敲除小鼠（EKO, GSE163657）与DKO小鼠的主动脉转录组，以演示功能富集结果聚合软件reString（https://github.com/Stemanz/restring）的使用方法。本研究针对DKO小鼠的完整主动脉开展RNA测序（RNA-seq），每个实验组设置3~4个生物学重复，测序平台为Illumina Genome Analyzer IIx。实验共包含2种饲料处理（常规饲料、西式高脂饲料）与2个时间节点（6周、22周）。