Hepatic transcriptomics reveals that lipogenesis is a key signaling pathway in isocitrate dehydrogenase 2 deficient mice

The present study was conducted to capture signatures of isocitrate dehydrogenase 2 deficient (Idh2-/-) mice liver transcriptome using mRNA sequencing technique which provides information to discover new transcripts and to quantify transcript levels. Using this mRNA sequencing results, bioinformatic analyses will be performed to further identify key biological functions of Idh2 influencing disease-related signaling pathways. Overall design: Hepatic mRNA profiles of wild type (WT) and Idh2-/- mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.