Locomotor behavior summary for <i>R6-Gal4</i> mediated RNAi knockdown of PDF.

The R6-Gal4 line showed relatively low levels of rhythmicity under DD conditions, but y w,uas-Dcr2;R6-Gal4/+ flies were robustly rhythmic. y w,uas-Dcr2/y;R6-Gal4/+;uas-PDFrnai/+ flies displayed a very high incidence of arhythmicity under DD conditions relative to control lines. ANOVA revealed significant difference in τ under DD (p<0.0001), but a Tukey's test revealed no significant difference in period between y w,uas-Dcr2/y;R6-Gal4/+;uas-PDFrnai/+ flies and y w,uas-Dcr2;R6-Gal4/+and y w,uas-Dcr2;;uas-PDFrnai/+ controls (p>0.05 for both comparisons). ANOVA revealed a significant difference in the power of free-running locomotor rhythms between genotypes, but a Tukey's test revealed no significant difference between between y w,uas-Dcr2/y;R6-Gal4/+;uas-PDFrnai/+ flies and y w,uas-Dcr2;R6-Gal4/+ controls (p>0.05). ANOVA revealed a significant difference in evening peak phase between genotypes and a Tukey's test revealed significant differences in phase between y w,uas-Dcr2/y;R6-Gal4/+;uas-PDFrnai/+ flies and y w,uas-Dcr2;R6-Gal4/+(p<0.01) and y w,uas-Dcr2;;uas-PDFrnai/+ controls (p<0.001). Tukey's test revealed no difference in evening peak phase between y w,uas-Dcr2/y;R6-Gal4/+;uas-PDFrnai/+ flies and R6-Gal4 controls.