Phytochrome B-SPA-COP1 regulation of red light induced gene expressions in Arabidopsis thaliana

The mRNA expression profiles of the quadruple spa mutant spaQ and cop1-4 were analyzed in 1 hour red light treated condition. Under same condition, the gene expression changes in phyB-SPA1 interaction domain mutant was analyzed and compared with wild type. Total fifteen 1h red light treated Arabidopsis samples were used. These include three replicates of following genotypes: Col-0, cop1-4, spaQ, phyB C-terminal deletion, phyB full length. Total six additional samples were used for dark control. These include three replicates of following genotypes: phyB C-terminal deletion, phyB full length.