Presence of acanthamoeba and diversified bacterialflora in poorly maintained contact lens cases.

Acanthamoeba is a free-living amoeba and is a representative pathogen that can cause keratitis. Acanthamoeba keratitis (AK) is a visually devastating disease and is refractory to conventional treatments. AK is known to have strong associations with poor contact lens (CL) and CL cases care. In this study, to examine whether a unique microbial community may be associated with the presence of acanthamoeba in CL cases, we performed a 16S rDNA sequencing analysis on illumina platform using highly contaminated CL cases.