Root transcriptome of CLE3 overexpressing lines and clv1-15 mutant lines

Nitrogen availability significantly impacts the development of plant root system architecture as plants must optimize growth for efficient acquisition of this nutrient. A signaling pathway involving the CLE family of small signaling peptides and the CLV1 leucine-rich repeat receptor kinase has been shown to repress lateral root development when plants are undergoing nitrogen starvation. Targets of this pathway remain unstudied and may be implicated in the coordination of N-responsive root growth. We obtained the clv1-15 mutant in Landsberg erecta (Ler) background and have generated transgenic lines overexpressing CLE3 in Columbia-0 (Col-0) background for further analysis. To identify downstream components of CLE-CLV1 signaling, we analyzed the transcriptome profiles of CLE3 overexpressing lines and the clv1-15 mutant. Root tissue from Landsberg erecta (Ler) and Columbia-0 (Col-0) wild-type accessions, two CLE3 overexpression lines in Col-0 background, and clv1-15 mutant in Ler background were used for this analysis. Plants were grown for 10 days on MGRL media supplemented with 0.1 mM KNO3. Experiments were performed in triplicate for Ler, Col-0, and clv1-15 and in duplicate for CLE3 overexpression lines. Samples were hybridized to the ATH1 Array (Affymetrix).