Decoupling the Biochemical and Mechanical Effects of Muscle Exercise on Motor Neurons with 2.5D Actuating Matrices [240614Ram_RNA-seq]

We wanted to query the differences and similarities between our wild-type C2C12 cell line and our optogenetic ChR2 C2C12 myoblasts on the same day of differentiation. After 6 days on muscle differentiation medium, we extracted the RNA and sequenced it for 6 wells each. Overall design: Transcriptomic comparison of wild-type C2C12 and ChR2-optogenetic C2C12 The O1-O6 samples were six wells of optogenetic C2C12 myoblast that were differentiated in high glucose medium with IGF and aminocaproic acid for six days prior to RNA extraction. Samples WT1-WT6 are the control samples which underwent the same culture conditions but using a WT line.