RNA-seq analysis of hepatocellular carcinoma (HCC), in which HBV-KMT2B integration was identified. The tumor region and nontumorous region were extracted from the HCC surgical specimen.

To investigate the transcriptomic analysis of HBV-KMT2B integrated HCC, RNA-seq analysis of tumor tissue of HBV-KMT2B integrated HCC was performed. To further analyze KMT2B integrations, we identified full-length complementary DNA by long-read RNA-seq (Iso-seq). Overall design: We investigated the transcriptomic analysis of HBV-KMT2B integrated HCC. To investigate HBV integration, we detected HBV-human junctions by next-generation sequencing. Three HBV-KMT2B integration were identified in 45 HBV-related HCC. the tumor and nontumorous tissues from one of the identified HCCs were obtained and extracted total RNA. For RNA-seq, tumor tissue from HBV-KMT2B integrated HCC were used.