Effect of interferon beta on the level of adipcoyte IRF7 and mitochiondrial content

Interferon regulatory factor 7 (IRF7) is an interferon-stimulated gene product. Interferons may damage mitochondria and increase IRF7 level. Our hypothesis was that higher IRF7 llevels may promote mitochondrial damage (reduce mitochondrial load of adipocytes), and IFNbeta may be the key trigger of incresed IRF7 level. Overall, we measured IRF7 protein level, IFNbeta level and mitochondrial content of primary mouse and human fat cells and 3T3-L1 cells.