overview_algae_assay.xlsx
收藏DataCite Commons2025-03-03 更新2025-04-17 收录
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A battery of four bioassays was exposed to the POCIS extracts to determine environmental mixture toxicity at the four sites (Table 1). Before application in the bioassays, the POCIS extracts were evaporated under a gentle stream of N<sub>2</sub> to dryness and reconstituted in dimethyl sulfoxide (DMSO). A whole organism (in vivo) algal bioassay was performed with the marine alga <i>Dunaliella tertiolecta</i> at the University of Amsterdam to assess photosynthetic inhibition (Sjollema et al., 2014) using pulse amplitude modulation (PAM) fluorometry according to de Baat et al. (2018). Dose-response curves for the in vivo assays were made in GraphPad Prism (GraphPad Software Inc., version 9.1.2.226, San Diego, CA, USA) and the half maximal effective concentration (EC<sub>50</sub>), expressed as the relative enrichment factor of the extract in the bioassays, was determined by non-linear regression with a built-in log-logistic model. Toxicity was expressed in toxic units (TU) and was calculated by 1/EC<sub>50</sub>.
提供机构：
University of Amsterdam / Amsterdam University of Applied Sciences
创建时间：
2025-03-03
