RNA seq of sorted MHCII positive cells from dorsal root ganglia under allergic contact dermatitis condition

Allergic contact dermatitis (ACD) is a common issue in occupational and environmental health (1), affecting over 25% of the general population aged 18-74 years old in Europe. It leads to substantial healthcare expenses and decreased work productivity. Current therapies for chronic itch associated with ACD have limitations, highlighting the need for new medications targeting key molecular pathways involving pruriceptive sensory nerve fibers and specific receptors for pruritogens.The interaction between sensory nerve fibers and cutaneous immune cells is crucial for itch sensation. These nerves can be activated by exogenous pruritogens and endogenous ones like histamine and inflammatory mediators . Understanding neuroimmune interaction could reveal new treatment targets for itch, but research on immune cells in the dorsal root ganglia (DRG) is still limited.In our previous research on toluene diisocyanate (TDI)-induced ACD using a mouse model, we observed an increase in the number of cells expressing major histocompatibility complex class II (MHCII) in DRG. However, there is still much to be understood about the function of these cells. Therefore, we conducted an analysis of transcriptomic changes in MHCII positive cells within DRG to gain further insights into their involvement in itch. The study involved sensitizing and challenging BALB/c mice with TDI, followed by collection of samples from three individual mice each from both control and TDI-sensitized groups. MHCII-positive cells were isolated from the DRG cell solution using fluorescence-activated cell sorting (FACS) for subsequent RNA sequencing analysis.