QTL analysis of the C24::AlSRKb-SCRb x Col-0 cross.

aThe NIL for QTL3.1 encompasses the small peak to the left of QTL3.1 shown in Figure 5. All NILs were tested for breakdown of SI by examining manually self-pollinated flowers over the course of floral development and by observation of seed set. NILs corresponding to QTL1, QTL3.1, and QTL5 exhibited no seed set and were SI at all developmental stages. NIL3.2, corresponding to QTL3.2, set noticeably more seed than the other NILs, though less than Col-0::AlSRKb-SCRb. A total of 29 developmental series of four consecutive manually self-pollinated flowers were used to assess NIL3.2. Only in three of those series was a weak breakdown of SI observed, and only in mature flowers.bThe Max LOD Score indicates the likelihood score directly under a QTL peak.