Regulation of major histocompatibility complex class-I (MHC-I) gene methylation using DNA methyltransferase inhibitor and PD-L1 inhibitor in triple negative breast cancer

The relationship between the expression of MHC-I and DNA methylation was analyzed using data from The Cancer Genome Atlas (TCGA). The expression of DNMTs and MHC-I was analyzed using qRT-PCR, and DNA methylation status of MHC-I genes was analyzed using methylation specific PCR in TNBC cell lines. TNBC cell lines were treated with DNMT inhibitors and programmed cell death-ligand 1 (PD-L1) inhibitor. Then, changes in the expression of DNMT and MHC-I, and methylation patterns of MHC-I genes were analyzed.