Transcription profiling by array of dorsal root ganglia (DRG) from conditional SNS-gp130-/- and control (gp130fl/fl) mice

To analyze the effect of Glycoprotein 130 in small diameter sensory neurons, dorsal root ganglia (DRG) of conditional SNS-gp130-/- and control (gp130fl/fl) mice were collected and mRNA expression profiles were compared between the two groups. DRG samples of two littermate mice were always pooled and a total of 10 gp130fl/fl and 10 SNS-gp130-/- mice were divided into 5 groups per genotype. Expression of transcripts were analyzed using the Applied Biosystems microarray platform AB1700 (Mouse Genome Survey Microarray V2.0).为分析糖蛋白130（Glycoprotein 130）在小直径感觉神经元中的作用，我们收集了条件性SNS-gp130基因敲除（SNS-gp130-/-）小鼠与对照（gp130fl/fl）小鼠的背根神经节（dorsal root ganglia, DRG），并对比两组样本的mRNA表达谱。实验中始终将2只同窝小鼠的背根神经节样本混合，每种基因型各取10只小鼠，按基因型分为5组。转录本的表达水平采用应用生物系统公司（Applied Biosystems）的AB1700小鼠基因组表达芯片V2.0（Mouse Genome Survey Microarray V2.0）平台进行分析。