Genetic confirmation of an “Uncommon Mourningthroat” (Geothlypis philadelphia x G. trichas): a rare but persistent hybrid warbler

For much of the history of American ornithology, hybrid birds discovered outside of known hybrid zones have presented an identification challenge—given the likely parental species are typically identified based on inferences from plumage patterns—but also a source of insight into evolutionary processes. With the advance of modern genetics, the tools are now available to definitively identify hybrids for which genetic samples are available and, in many cases, determine the identity and the sex of each parent species as well as F1 versus backcross status. Typically, these kinds of hybrids are rare; however, one apparently common hybrid wood-warbler (family Parulidae) are putative crosses between Mourning Warblers (Geothlypis philadelphia) and Common Yellowthroats (G. trichas), which have been reported at least thirteen times between 1955 and 2024, though never confirmed genetically. Here we describe the results of genetic and plumage analysis to identify a putative G. philadelphia x G. tr..., , # Genetic confirmation of an “Uncommon Mourningthroat” (Geothlypis philadelphia x G. trichas): a rare but persistent hybrid warbler

Dataset DOI: [10.5061/dryad.nvx0k6f3n](10.5061/dryad.nvx0k6f3n)

## Description of the data and file structure

### Data

* bam.list - Example input to to PCAngsd, sample order is unchanged.

* bam.2.list - Example input to NGSAdmix, sample order is unchanged. Both bamlist sample names follow those found in NCBI BioProject PRJNA630247.

* moye.gl1.cov - The covariance matrix, output of PCAngsd. The eigenvectors can be calculated using the eigen() command in R. The order of individuals is the same as bam.list.

* moye_maf0.2_k2_gl1.txt - Admixture proportions of each individual. The order is the same as bam.2.list.

* sanger1.ab1 - The forward Sanger trace.

* sanger2.ab1 - The reverse Sanger trace.

* geothlypis_coi_unaligned.fasta - The COI sequences from the hybrid and each Geothlypis/Oporornis species as downloaded from GenBank.

* geothlypis_co...,