Pathogenic pathways in early onset autosomal recessive Parkinson's disease discovered using isogenic human dopaminergic neurons

Purpose of NGS transcriptomics: We generated RNA-sequencing transcriptomics datasets from isogenic Parkinson's disease (PD) HUES1 cell lines (harboring loss of function mutations in either PARKIN (PRKN), DJ-1-/- (PARK7), or ATP13A2-/- (PARK9)) to identify common and distinct dysregulated genes and networks in our three isogenic PD lines in an unbiased way, with the goal of grouping similar and distinct forms of PD and identify common or divergent dysregulation. Overall design: Transcriptional profiles of 12 samples (3 replicates of 4 isogenic cell lines) were generated after sorting hPSC derived dopaminergic neurons using Tyrosine Hydroxylase knock-in fluroescence reporter