Molecular mechanism for the synchronized electrostatic coacervation and co-aggregation of alpha-synuclein and tau

<strong><em>The following metadata refers exclusively to electron paramagnetic resonance (EPR) measurements, which represent the contribution of the PARACAT students to this work</em></strong> <strong>Data type</strong>: EPR spectroscopic measurements and simulations Files are in <strong>.DTA, .DSC, .m, .mat, and .xlxs, </strong>formats Information on <strong>origin of the data</strong>: EPR spectroscopic measurements in <strong>.DTA </strong>and<strong> .DSC</strong> formats EPR spectroscopic simulation and analyses in .<strong>m </strong>and<strong> .mat</strong> format “Ready-to-plot”, processed EPR spectra are in <strong>.xlxs</strong> format. The data are <strong>generated</strong> by: CW-EPR measurements were performed with a Bruker ELEXSYS E580 X-band spectrometer equipped with a Bruker ER4118 SPT-N1 resonator operating at a microwave (MW) frequency of ∼9.7 GHz. The temperature was set to 25 °C and controlled by a liquid nitrogen cryostat. <strong>If the dataset includes multiple files that relate to each other:</strong> Files in <strong>PARACAT_WP3_20221219_EPR </strong>folder includes EPR spectroscopic measurements and computer simulations/analyses, original data are in <strong> .DTA/.DSC</strong> formats; files in .<strong>m</strong> format were used to process the data. NB. See the “READ ME” text file for more detailed information on files organization. <strong>Information on</strong>: Abbreviations: <strong>avg</strong> = averaged <strong>aS_24</strong> = alpha-synuclein protein with TEMPOL spin label at position 24 of the polypeptidic chain <strong>aS_122</strong> = alpha-synuclein protein with TEMPOL spin label at position 122 of the polypeptidic chain <strong>pLK</strong> = poly-lysine <strong>Tau441</strong> = Tau protein with complete amino-acid sequence <strong>Tau_DNt</strong> = truncated Tau protein lacking N-terminal (see paper methods for further details) Units of measurement: Temperature: <strong>°</strong><strong>C</strong> (Celsius) Microwave Frequency: <strong>GHz</strong> (Giga-Hertz), <strong>MHz</strong> (Mega-Hertz), <strong>kHz</strong> (kilo-Hertz) Microwave Power: <strong>mW</strong> (milli-Watt) Magnetic Field: <strong>mT</strong> (milli-Tesla) Time: <strong>s</strong> (seconds) Concentration: <strong>μM</strong> (micro-Molar), <strong>% w/v</strong> (percentage weight-volume)