Oryza sativa cultivar:Huayoung Raw sequence reads. Oryza sativa cultivar:Huayoung

Soil salinity is a severe obstacle for crop growth and production that limits the growing food demands. Circadian clock confers plant fitness advantage by regulating manyaspects of growth and development. But how circadian regulate salt stress in riceis well unknown. Here, we characterized the OsELF4.1, a member of rice (Oryza sativa L.) circadian clock, which is involved in responding to salt stress and crop production, providing a target to research the balance maintaining between growth and stress tolerance simultaneously. Co-immunoprecipitation analysis confirmed that OsELF4.1 is able to form a complex with OsELF3.1 and OsLUX, which is found to be required for salttolerance. RNA-seq data further reveal that OsELF4.1 involves in stress response pathway and abiotic stimulus response pathway, consistent with salt sensitive phenotype of oself4.1, oself3.1 and oslux mutant. By chromatin immunoprecipitation (ChIP) quantitative PCR assays, we found that OsELF4.1 binding the promoter of GI which is a major gene in regulating salt tolerance.