List of differentially expressed genes between keratinocytes treated with SPD versus non-treated controls

These data summarize acute transcriptomic effects of 6-hour 10 µM spermidine (SPD) treatments versus non-treated controls (n=6 per condition). For these experiments, 6-well treatment plates were removed from incubators, treatment media was decanted, cells were washed with PBS, and 500 µL of Trizol (Thermo Fisher Scientific; Waltham, MS, USA) and RNA isolation was performed using chloroform, aqueous phase isolation and isopropanol precipitation per manufacturer’s instructions. RNA pellets were resuspended in 30 µL of RNase-free water, concentrations were analyzed in duplicate using a Nanodrop Lite spectrophotometer (Thermo Fisher Scientific). RNA was then frozen at -80ºC and shipped to a commercial laboratory (North American Genomics, Decatur, GA, USA) on dry ice for transcriptomic analysis using the Clariom S Assay_Human mRNA array (Thermo Fisher Scientific). Prior to analyses, RNA quality was confirmed using a commercially available assay (Agilent, Santa Clara, CA, USA, catalog #: 5067-1511) measured with the Agilent 2100 Bioanalyzer system. Raw transcriptomic data were received as .CEL files and analyzed using the Transcriptome Analysis Console v4.0.2 (Thermo Fisher Scientific). The hg38 (h. sapiens) genome was used for annotations, data were normalized using the robust multiarray average (RMA) normalization method, and transcripts are presented as log2 signal intensities. Significance thresholds were defined a priori as p<0.01 as we have previously implemented with mRNA arrays.