Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro

We investigated the effect of cyclic tensile stresses on cultured spinal cord cells from E15 Sprague-Dawley rats, using the FX3000® Flexercell Strain Unit. We examined cell morphology and viability over a 72 hour time course. Microarray analysis of gene expression was performed using the Affymetrix GeneChip System®, where categorization of identified genes was performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems.本研究采用FX3000® Flexercell应力加载装置，探究了周期性拉伸应力对15天胚胎期斯普拉-道来大鼠（E15 Sprague-Dawley rats）体外培养脊髓细胞的影响。随后在72小时的时间进程中，检测了细胞的形态学特征与存活活性。本研究借助Affymetrix GeneChip System®开展基因表达的微阵列分析，并通过基因本体（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）系统对鉴定得到的基因进行功能分类。