The specificity of gene activation was revealed by RNA-seq in the HEK293T cells transfected with the dCasMINI-VPR or dhpCasMINI-VPR system targeting IL1RN [RNA-seq]

The specificity of gene activation, both the dCasMINI-VPR and dhpCasMINI-VPR significantly and obviously activated IL1RN and only a few genes showed expression change, indicating high genome-wide targeted transcriptional specificity. Overall design: We performed transcriptome profiling by RNA-seq in HEK293T cells co-transfected with the dCasMINI-VPR or dhpCasMINI-VPR system targeting IL1RN for assessing the gene activation specificity. The total amount of DNA was 500ng per well for a 24-well plate, and plasmids encoding the dCasMINI-VPR or dhpCasMINI-VPR and sgRNA were transfected at a 1:49 ratio. Approximately 1 × 105 cells were plated in 24-well plates 1 day before transfection, and analyzed 2 days post-transfection for transcriptome profiling by RNA-seq.