Expression of neural stem cell markers in mouse ADAS grown in Mesencult and exposed to Neural Induction Medium (NIM).

Mouse ADAS cells were proliferated in Mesencult and exposed to NIM for the times indicated prior to harvest in 4% paraformaldehyde for immunocytochemical analyses. Semi-quantitative assessment of marker expression was performed using the following criteria: +++ = robust expression in greater than 50% of the cells; ++ = moderate expression in greater than 50% of the cells; + = low expression in more than 50% of the cells; +/− = low expression in less than 50% of the cells; − = no detectable expression. Asterisk (*) indicates marker expression confirmed by real-time PCR. Abbreviations: NIM, Neural Induction Medium; NSC, Neural Stem Cell; NSE, Neuron Specific Enolase; GFAP, Glial fibrillary acidic protein; Tuj1, β-tubulin III