Lepr^em4Lizh

The Lepr knockout mutation were generated by CRISPR/Cas9. The resulting mutaion is a 298-bp deletion from No. 90043&#8201;bp to 90341&#8201;bp in the Lepr genome DNA sequence (NC_005104.4) and a 4-bp insertion and resulted in a termination codon TGA. For genotyping, a 662-bp fragment of WT and a 368-bp fragment of the Lepr knockout gene were amplified with PCR.<br>ASSOCIATED WITH decreased bone mineral density; decreased trabecular bone volume; expanded mesangial matrix; ASSOCIATED WITH Dyslipidemias; glucose intolerance; hyperglycemia通过CRISPR/Cas9技术构建的Lepr敲除突变体。该突变导致Lepr基因DNA序列（NC_005104.4）中第90043碱基至第90341碱基之间发生298碱基的缺失，并伴随4碱基的插入，最终导致终止密码子TGA的出现。为了进行基因分型，利用聚合酶链反应（PCR）扩增了野生型基因的662碱基片段和Lepr敲除基因的368碱基片段。该突变与降低的骨矿物质密度、减少的骨小梁骨体积、扩大的系膜基质有关，并伴随有血脂异常、葡萄糖不耐受和高血糖等病症。