5P-Seq analysis of wt and new1-knockout yeast strain as well as New1:80S pull-down samples

We present two sets of 5P-Seq experiments: i) comparison of wt and new1-KO S. cerevisiae ii) analysis of New1:80S pulldown samples. Collectively our results unravel codon-dependent nature of ribosomal stalling in S. cerevisiae lacking New1.本研究报道了两组5P-Seq（5'磷酸化末端测序）实验：其一为野生型（wild type，wt）与new1基因敲除酿酒酵母（S. cerevisiae）的对照实验；其二为New1:80S核糖体下拉样本的分析实验。综上，本研究结果揭示了缺失New1的酿酒酵母中核糖体停滞的密码子依赖性本质。