Bulk RNA-seq of murine ?dT17 cells isolated from the lamina propria of Tbet-deficient mice

This experiment was designed to explore the biological relevance of the transcription factor Tbet in intestinal ?dT17 cells. For this purpose, we crossed TbetF/F with ROR?tCRE-R26LSLRFP mice, where RFP expression in the ROSA26 locus was induced by ROR?t-driven Cre recombinase. We then sorted Tbet-deficient ?dT17 cells (ROR?tRFP+) from lamina propria and performed bulk RNA-seq.