Microbiome analysis of digested pig slurry, and anode biofilms of MEC, with DNA and RNA extraction

The main aim of this study is (1) to assess the combination of the anaerobic digestion (AD) process with a microbial electrolysis cell (MEC) with a recirculation loop as a system to remediate AD reactors that have experienced process failure, and (2) study the microbial population in an AD-MEC system during inhibited and recovered states of the AD process, regarding presence of eubacteria and archaea in the biomass, but also regarding gene expression by means of DNA and RNA extraction, sequencing and quantification.