Transcriptomics of the Human Hypothalamus across the FTD-MND Spectrum

This dataset contains raw count data, derivatives (as Seurat objects), and analysis result tables (supplementary tables for upcoming thesis and manuscripts) derived from single-nucleus RNA sequencing and spatial transcriptomics experiments. Tissues were collected from the Sydney Brain Bank and consist of hypothalamus tissues from donors on the frontotemporal dementia (FTD) and motor neuron disease (MND) spectrum. Single-nuclei (Chromium Flex for FFPE) data was derived from 3 donors with MND, 3 donors with behavioural-variant FTD (bvFTD), and 2 donors with FTD-MND. Spatial transcriptomics (Visium HD for FFPE) was conducted on two donors with bvFTD.This dataset contains: derivative data (Seurat objects in .rds format)raw single-nuclei counts from 10x Chromium (CellRanger v7.2.0 output)analysis result tables (.xlsx)segmented counts from Visium HD (SpaceRanger v4 output) The generation of this data is supported by a Royal Brisbane and Women’s Hospital Foundation (private donation), Motor Neurone Disease Research Australia (NTI MND Research Grant and Murray Geale Research Grant), and the Brain Foundation.