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GNPS - C.acnes and S.aureus BHI coculture Pre_XAD

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NIAID Data Ecosystem2026-03-11 收录
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Monoculture and coculture of Cutibacterium acnes KPA171202 and Staphylococcus aureus JE2 on BHI liquid medium containing 100uL XAD16. Anaerobic culture conditions for 3-5 days. Cultures were centrifuged and supernatant removed. XAD16 slurry removed and extracted with 100%MeOH. Extract was freeze-dried and resuspended for LC-MS/MS performed in an UltiMate 3000 UPLC system (Thermo Scientific) using a Kinetex 1.7 mm C18 reversed phase UHPLC column (50 X 2.1 mm) and Maxis Q-TOF mass spectrometer (Bruker Daltonics) equipped with ESI source.

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2019-12-11
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