Table_1_Gut Microbiota of Chinese Obese Children and Adolescents With and Without Insulin Resistance.docx

ObjectiveThe intestinal flora of gut microbiota in obese Chinese children and adolescents with and without insulin resistance (IR) was analyzed, as well as associations between the gut microbiota and two serum cytokines related to glucose metabolism, adropin and angiopoietin-like 4 (ANGPTL4).MethodsClinical data, fecal bacterial composition, glucose-related hormones, and serum adipokines (adropin and ANGPTL4) were analyzed in 65 Chinese children with exogenous obesity. The composition of the gut microbiota was determined by 16S rRNA-based metagenomics and IR was calculated using the homeostasis model assessment (HOMA).ResultsThe 65 obese subjects were divided into two groups: insulin sensitive (IS) (n=40, 57.5% males) or IR (n=25, 60% males). Principal coordinates analysis revealed that the gut microbiota samples from the IS group clustered together and separated partly from the IR group (p=0.008). By Mann-Whitney U-test, at a phylum level, a reduction of Firmicutes and an increase of Bacteroidetes in the IR subjects was observed. LEfSe analysis revealed that IS subject, when compared to their IR counterparts, harbored members of the order Coriobacteriales, Turicibacterales, Pasteurellales and family Turicibacteraceae, that were significantly more abundant. In contrast, the IR subjects had members of family Peptococcaceae that were significantly more prevalent than the IS subjects (all p

研究旨在分析肥胖的中国儿童和青少年肠道菌群，包括是否存在胰岛素抵抗（IR）的情况，并探讨肠道菌群与两种与葡萄糖代谢相关的血清细胞因子——腺肽和血管生成素样蛋白4（ANGPTL4）之间的关联。研究方法包括对65名外源性肥胖的中国儿童的临床数据、粪便细菌组成、与葡萄糖相关的激素以及血清脂肪因子（腺肽和ANGPTL4）进行分析。通过基于16S rRNA的宏基因组学方法确定肠道菌群的组成，并利用稳态模型评估法（HOMA）计算胰岛素抵抗。研究结果显示，65名肥胖受试者被分为两组：胰岛素敏感组（IS组，n=40，男性占比57.5%）和胰岛素抵抗组（IR组，n=25，男性占比60%）。主坐标分析显示，IS组的肠道菌群样本聚集在一起，并且部分与IR组分离（p=0.008）。通过Mann-Whitney U检验，在门水平上，IR组中厚壁菌门减少，拟杆菌门增加。LEfSe分析显示，与IR组相比，IS组拥有显著更多丰度的属于芽孢杆菌目、土伦菌目、巴氏菌目和土伦菌科成员，而IR组中佩托科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科科