The role and function of YAP in cancer-associated fibroblasts

To investigate how YAP activity contributes to the functional properties of cancer-associated fibroblasts (CAFs), we established YAP-deficient cancer-associated fibroblast cell line, in which YAP expression can be inducibly silenced using shRNA, for RNA-Seq compared to wildtype CAFs. Overall design: Total RNA was extracted from both wildtype CAFs and YAP-deficient CAFs for RNA-Seq. RNA-Seq data were analyzed using DESeq2 on the AIR platform (https://transcriptomics.sequentiabiotech.com/).