RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae

Verticillium dahliae is a soilborne fungus that causes wilt disease in plants. The microsclerotia of V. dahliae produce infectious hyphae that give rise to primary infections. In this study, RNA-seq libraries were prepared from microsclerotia (MS)-producing cultures of V. dahliae (ave = 52.23 million reads), and those not producing microsclerotia (NoMS, ave = 50.58 million reads) and analyzed for differential gene expression.

大丽轮枝菌（Verticillium dahliae）是一种可引发植物萎蔫病的土传真菌。该菌的微菌核（microsclerotia）可萌发形成侵染性菌丝，进而引发初侵染。本研究从产微菌核（简称MS）的大丽轮枝菌培养物（平均测序读段数为52.23百万）以及不产微菌核（简称NoMS）的培养物（平均测序读段数为50.58百万）中构建RNA测序（RNA-seq）文库，并对两组样本的差异基因表达情况进行分析。

搜集汇总

背景与挑战

背景概述

该数据集通过RNA-seq技术分析Verticillium dahliae真菌微菌核形成过程中的基因表达差异，比较了微菌核产生（MS）和非产生（NoMS）培养物的转录组数据，平均读取数超过5000万。数据集包含6个实验，总数据量达31 Gbases，专注于单分离株的转录组研究，旨在揭示该植物病原真菌的感染机制。