Transcript analysis under calcium deficiency in 6 day old Marchantia polymorpha wildtype (Tak-1) and Gß-null mutant (gpb1-2) gemmalings and inter-species comparison of M. polymorpha, Arabidopsis and lettuce under calcium deficiency

Calcium deficiency response in liverwort, Arabidopsis and lettuce: (1) Marchantia polymorpha: M. polymorpha wildtype and Gß-null mutant plants (Tak-1, gpb1-2) were grown in control liquid Yamagami media (2 mM Ca) for 6 days. For RNA-seq experiments, 6 day old gemmalings were transferred to calcium deficiency (0 mM Ca) media. Samples were collected at 48 h after the transfer. The transcriptomic profiles were collected from two independent batches. In total four biological replicates were used for each condition and each genotype for a total of 16 samples. (2) Arabidopsis thaliana: For Arabidopsis RNA-seq experiment, 6-day old seedlings grown on ½ strength MS media with sucrose were transferred to Yamagami media with 2 mM or 0 mM CaCl2 and treated for 7 days. (3) Lactuca Sativa: For lettuce RNA-seq, 4-day old seedlings grown on water agar (1%) were transferred to Yamagami media with 2 mM or 0.15 mM CaCl2 and treated for 7 days. In total four and three biological replicates were used for each condition for a total of 8 and 6 samples respectively for Arabidopsis and lettuce. Overall design: Transcript analysis of 6-day old M. polymorpha gemmalings under two conditions (2 mM Ca, 0 mM Ca) with wild type Tak-1 and gpb1-2. Transcript analysis of 6-day old (Arabidopsis) under two conditions (2 mM Ca, 0 mM Ca) and 4-day old (lettuce) wildtype seedlings under two conditions (2 mM Ca, 0.15 mM Ca).