Sequencing of intestinal flora in mice with gallbladder stones at different altitudes based on 16S rDNA research

Objective: To establish a gallstone mouse model using a lithogenic diet and investigate changes in the gut microbiota of gallstone mice at different altitudes.Methods: Sixty mice were randomly assigned to four groups: plain healthy, plain stone, high-altitude healthy, and high-altitude stone. Mice were raised in either plain or high-altitude environments, and a lithogenic diet was used to induce gallstone formation. After 8 weeks, the mice were euthanized, and stone formation was assessed. Fecal samples were also collected for 16S rDNA high-throughput sequencing to analyze the gut microbiota.