The effect of gut microbiota on the prognosis of type 2 diabetes mellitus complicated with circadian clock disorder

Stool samples were collected from six patients with poorly controlled type 2 diabetes mellitus (T2DM) and circadian rhythm disruption, as well as from six patients with well-controlled T2DM and circadian rhythm disruption. Metagenomic sequencing was performed on the stool samples. Compared to the well-controlled group, the abundance of Lactobacillus johnsonii was significantly decreased in the poorly controlled group. To investigate the effects of L. johnsonii supplementation on glucose and lipid metabolism, diabetic mice with circadian rhythm disruption were administered L. johnsonii and their metabolic indicators were measured.