Microarray expression profiling in the denervated hippocampus identifies long noncoding RNAs functionally involved in neurogenesis

RNA samples were extracted from the paired rat untreated hippocampus 7 days after the FF transection. The general RNA quality was evaluated by RIN. RNA quantity and purity were assessed using K5500 micro-spectrophotometer. A260/A280 =1.5 and A260/A230 =1 indicated acceptable RNA purity, and RIN value =7 using the Agilent 2200 RNA assay indicated acceptable RNA integrity (Table. S1). Genomic DNA contamination was evaluated by gel electrophoresis .Three independent samples were assayed to evaluate the reproducibility of the experimental procedure. Using the microarray expression profiles, 103 (74 activated and 29 repressed) lncRNAs differentially expressed in the hippocampus after the FF transection were identified.本研究提取的RNA样本，均来自FF切断术后7天的配对未处理大鼠海马组织。总RNA的整体质量通过RNA完整性指数（RIN）进行评估；RNA的总量与纯度采用K5500微量分光光度计（K5500 micro-spectrophotometer）检测：当A260/A280比值为1.5且A260/A230比值为1时，判定RNA纯度合格；采用安捷伦2200 RNA检测系统（Agilent 2200 RNA assay）测得RIN值为7时，判定RNA完整性合格（详见补充表S1）。基因组DNA污染情况通过凝胶电泳进行评估。本研究设置3份独立样本进行检测，以验证实验流程的可重复性。基于芯片表达谱分析，共鉴定出103个在FF切断术后海马组织中差异表达的长链非编码RNA（lncRNAs），其中74个为上调表达，29个为下调表达。