Real-time RT-PCR for pmcA-C genes from the in vivo microarray.

*The mRNA abundance of A. fumigatus pmcA-C genes during growth in lung alveoli. Real-time RT-PCR was used to quantify mRNA abundance. The measured quantity of mRNA for a specific gene in each of the treated samples was normalized using the CT values obtained for the β-tubulin mRNA amplifications run on the same plate. The relative quantitation of a specific gene and β-tubulin gene expression was determined by a standard curve (i.e., CT –values plotted against a logarithm of the DNA copy number). The results of four sets of experiments were combined for each determination; means ± standard deviation are shown. The values represent the cDNA concentration of a specific gene divided by the β-tubulin cDNA concentration.