A single cell atlas of the mouse seminal vesicle

During mammalian reproduction, sperm are delivered to the female reproductive tract bathed in a complex medium known as seminal fluid, which plays key roles in signaling to the female reproductive tract and in nourishing sperm for their onwards journey. The majority of seminal fluid is produced by a somewhat understudied organ known as the seminal vesicle. Here, we report a single-cell RNA-Seq atlas of the mouse seminal vesicle, generated using tissues obtained from 23 mice of varying ages, exposed to a range of dietary challenges. We define the transcriptome of the secretory epithelial cells in this tissue, identifying a relative homogeneous population of the epithelial cells responsible for producing the majority of seminal fluid. We also define the immune cell populations – including large populations of macrophages, dendritic cells, T cells, and NKT cells – which have the potential to play roles in producing various immune mediators present in seminal plasma. Together, our data provide a resource for understanding the composition of an understudied reproductive tissue with potential implications for paternal control of offspring development and metabolism. Seminal vesicles were collected from FVB/NJ and C57BL/6J mice at age of about 12 weeks to 28 months. The animals were maintained under controlled temperature and humidity conditions on a 12 hr light/dark cycle with different diets and water ad libitum. The diets used included Chow diet, High Fat diet. Low Protein diet or AIN-93G (Control diet, CD). The mice on HFD and CD were fed for 9 weeks after weaning. Chromium Single Cell 3’ Reagent Kit V2 (10X Genomics) was used for single-cell sequencing libraries.