polysome profiling RNA seq experiments on extracts from METTL3-WT and METTL3-K177Q reconstituting cells
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https://www.ncbi.nlm.nih.gov/sra/SRP334680
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Given the overall steady-state mRNA abundance between these two groups displayed negligible differences, these data implied an increased translational efficiency in cells bearing K177Q mutation. Overall design: Using sucrose gradient centrifugation, collected fractions were pulled into sub-polysome fraction and polysome fraction. Then, sub-polysome and polysome samples were subjected to RNA-seq.
创建时间:
2022-11-11



