Gene expression profile at single cell level of stromal vascular cells (SVC) from the subcutaneous adipose tissue (SAT) and the visceral adipose tissue (VAT) post-exposure to SARS-CoV-2 or mock conditions in vitro

Obesity, characterized by chronic low-grade inflammation of the adipose tissue, is associated with adverse coronavirus disease 2019 (COVID-19) outcomes, yet the underlying mechanism is unknown. To explore whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of adipose tissue contributes to pathogenesis, we evaluated COVID-19 autopsy cases and deeply profiled the response of adipose tissue to SARS-CoV-2 infection in vitro. Overall design: SAT SVC and VAT SVC were isolated from 3 participants, exposed to either SARS-CoV-2 or mock conditions in vitro for 24 hours and analyzed using scRNAseq.