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Chromium 10X single-cell RNA-seq of iPSC-derived gut organoids

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP157687
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In this study we present a rapid and robust protocol for generating a responsive and transplantable model of colonic mucosa from hiPSCs. To characterise the cellular populations in the epithelial and mesenchymal compartments, single-cell mRNA sequencing was performed on sorted day 8 and day 15 cells. Four samples were sequenced: 1) day 8 EpCAM+ (epithelical) cells; 2) day 8 EpCAM- (mesenchymal) cells; 3) day 15 EpCAM- (mesenchymal) cells cultured with purmorphamine; 4) day 15 EpCAM- (mesenchymal) cells cultured without purmorphamine.
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2025-01-02
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