Chromium 10X single-cell RNA-seq of iPSC-derived gut organoids

In this study we present a rapid and robust protocol for generating a responsive and transplantable model of colonic mucosa from hiPSCs. To characterise the cellular populations in the epithelial and mesenchymal compartments, single-cell mRNA sequencing was performed on sorted day 8 and day 15 cells. Four samples were sequenced: 1) day 8 EpCAM+ (epithelical) cells; 2) day 8 EpCAM- (mesenchymal) cells; 3) day 15 EpCAM- (mesenchymal) cells cultured with purmorphamine; 4) day 15 EpCAM- (mesenchymal) cells cultured without purmorphamine.