Transcriptomic analysis to analyze gene expression of bacteria corroding iron

To identify the mechanism of Microbial Influenced Corrosion (MIC) and the bacterial response toward corrosion, we conducted whole genome microarray expression profile. At log phase, the cell of Clostridium carboxidivorans using iron granule as an electron donor (corroding iron) was collected as a sample, and that of using syngas as an electron donor was collected as a control. Two sets of gene expression, which were C. carboxidivorans extracting iron from iron granule and syngas was measeured. Four independent experiments were performed using different cultures.