A human single cell atlas of the substantia nigra reveals novel cell specific pathways associated with the genetic risk of Parkinson’s disease and neuropsychiatric disorders.

A study of the first human single-nuclei transcriptomic atlas for substantia nigra (SN), generated by sequencing ~ 17,000 nuclei from matched cortical and SN samples. Overall design: Single nuclei were extracted and isolated from 3mm2 blocks that were manually dissected from the substantia nigra and cortex of 5 control (4 males & 1Female) de-identified post-mortem human donors, totaling to 12 matched samples, including 2 SN replicates and sequenced using the 10X chromium system (V2).