Vulval and uterine muscle pattern in unc-37(RNAi) animals.

Expression of egl-15::gfp and rgs-2::gfp were used to score vm1 muscles and uterine muscles, respectively. egl-15::gfp expression is occasionally mosaic in wild-type and mutant animals. Animals containing four egl-15::gfp expressing vm1 vulval muscles were scored as wild type. It was difficult to count rgs-2::gfp expressing uterine muscles, so only animals with no rgs-2::gfp expression in the uterine muscles were scored as mutant.1ayIs2[egl-15::gfp] or vsIs5[rgs-2::gfp] animals were used.2Refers to animals with four cells expressing GFP (egl-15::gfp) or any GFP expression around the vulva (rgs-2::gfp).3L4440 is the RNAi feeding vector containing no insert [40].