Single-nuclei RNA Sequencing Reveals Distinct Transcriptomic Signatures of Rat Dorsal Root Ganglia in a Disc-associated Chronic Low Back Pain Model

This study presents the first application of comprehensive single-nuclei RNA sequencing (snRNA-seq) of dorsal root ganglion (DRG) neurons in a rat model using Parse technology. Our dataset includes two experimental models: (1) control rats representing both sexes and (2) rats with disc-associated chronic lower back pain, alongside sham-treated animals. By leveraging high-resolution transcriptomic profiling, this study provides novel insights into the molecular landscape of DRG neurons, offering a valuable resource for understanding the cellular mechanisms underlying chronic pain.本研究首次采用Parse技术，在大鼠模型中开展背根神经节（dorsal root ganglion, DRG）神经元的全面单细胞核RNA测序（single-nuclei RNA sequencing, snRNA-seq）分析。本数据集涵盖两类实验模型：（1）覆盖雌雄两性的正常对照大鼠；（2）椎间盘相关性慢性下腰痛模型大鼠，以及假手术处理组动物。本研究借助高分辨率转录组分析，为背根神经节神经元的分子特征全景提供了全新见解，同时为解析慢性疼痛的细胞机制提供了宝贵的科研资源。