MCF7 and MDA-MB-231 RNA-seq

LCOR is a corepressor of activated nuclear receptors that inhibits the transcriptional activity of these proteins. Recently, LCOR was described to act also as a transcription activator by DNA direct binding through its HTH domain of genes involved in antigen presentation. This double transcriptional role of LCOR is determined by the expression of nuclear receptor. We overexpressed LCOR and performed RNA-seq in breast cancer cell lines of nuclear receptor positive subtype (MCF7) and nuclear receptor absent (MDA-MB-231). This analysis allows to compare transcriptional regulation of LCOR depending on the presence of nuclear receptors. Overall design: MCF7 and MDA-MB-231 were transduced with inducible LCOR construct. After antibiotic selection, cells were treated with vehicle or 1 ug/ml of doxycycline. After 24 h of induction, cells were lysed following Qiagen RNA extraction protocol. RNA was submitted for RNA-sequencing and study the transcriptional changes occurring when LCOR is overexpressed in both breast cancer cell lines.